Fig. 2.

CCDC41 depletion prevents primary cilia formation. (A) Western blot illustrating efficient depletions of CCDC41 in RPE1 cells by each of three distinct siRNAs. Number sign indicates nonspecific bands. (B) RPE1 cells were shifted from 10% serum to serum-free media 48 h after siRNA transfection and cultured for an additional 24 h. Ciliated cells were identified by double labeling with anti–glu-Tub and anti–γ-Tub antibodies. Error bars represent SD (n = 3 independent experiments; *P < 0.01, t test). (C) CCDC41 knockdown efficiently inhibits ciliogenesis in most transfected cells without causing apparent changes in the appendage components Cep164 and Odf2. (Scale bar: 10 μm.)