Table 1.
Baseplate | BppL | Tripod short | Tripod long | Epitope | |
KD values (nM) BPTP901-1 nanobodies | |||||
Nanobody 2 | 10 ± 2 | 15 ± 3 | 13 ± 3 | 10 ± 4 | BppL |
Nanobody 11 | 19 ± 3 | 13 ± 1 | 20 ± 2 | 19 ± 3 | BppL |
Nanobody 17 | 300 ± 10 | 35 ± 3 | Nd | 20.6 ± 1 | BppL |
Nanobody 19 | 12 ± 2 | 12 ± 2 | 15 ± 2 | 7 ± 2 | BppL |
Nanobody 9 | 29 ± 3 | — | 1,000 ± 500 | 120 ± 40 | BppU Nt (+Ct) |
Nanobody 13 | 48 ± 8 | — | 64 ± 7 | 92 ± 11 | BppU Ct |
Nanobody 18 | 22 ± 2 | — | 280 ± 40 | 65 ± 9 | BppU Nt (+Ct) |
Nanobody 3 | 420 ± 50 | — | — | — | Dit |
Nanobody 7 | 170 ± 15 | — | — | — | Dit |
KD values (nM) Tal-Nt nanobodies | Isolated Tal-Nt 1–386 | ||||
Nanobody T11 | 32 ± 2 | ||||
Nanobody T41 | 30 ± 2 |
The measurements have been performed in triplicate. Nd, not determined.