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. 2013 Mar 25;110(15):E1371–E1379. doi: 10.1073/pnas.1301336110

Table 1.

Analysis by SPR of the interactions between nanobodies and phage TP901-1–expressed components

Baseplate BppL Tripod short Tripod long Epitope
KD values (nM) BPTP901-1 nanobodies
 Nanobody 2 10 ± 2 15 ± 3 13 ± 3 10 ± 4 BppL
 Nanobody 11 19 ± 3 13 ± 1 20 ± 2 19 ± 3 BppL
 Nanobody 17 300 ± 10 35 ± 3 Nd 20.6 ± 1 BppL
 Nanobody 19 12 ± 2 12 ± 2 15 ± 2 7 ± 2 BppL
 Nanobody 9 29 ± 3 1,000 ± 500 120 ± 40 BppU Nt (+Ct)
 Nanobody 13 48 ± 8 64 ± 7 92 ± 11 BppU Ct
 Nanobody 18 22 ± 2 280 ± 40 65 ± 9 BppU Nt (+Ct)
 Nanobody 3 420 ± 50 Dit
 Nanobody 7 170 ± 15 Dit
KD values (nM) Tal-Nt nanobodies Isolated Tal-Nt 1–386
 Nanobody T11 32 ± 2
 Nanobody T41 30 ± 2

The measurements have been performed in triplicate. Nd, not determined.