Fig. 3.

Differential expression of CXCR4 in SKNAS iCSC and monolayer cell xenografts. (A) The expression of CXCR4 and MYC was examined by TaqMan qPCR in SKNAS monolayer cell and iCSC xenografts, as described in Fig. 1. SKNAS monolayer cells and the in vitro culture of SKNAS iCSCs (day 175) were included as controls. Expression levels of the genes were presented as fold change over SKNAS monolayer cells in SKNAS iCSCs at day 175, SKNAS monolayer cell xenografts, and SKNAS iCSC xenografts. (B) Immunohistochemical analysis showed that SKNAS iCSC xenografts were uniformly positive for CXCR4. In contrast, SKNAS monolayer cell xenografts were negative for CXCR4, with the exception of some rare cases in which a few cells were focally positive for CXCR4 staining.