Table 1.
P. yoelii 18S rRNA liver-stage burden in GAP-immunized BALB/c mice
| Interval, d | Sporozoite doses | ΔCT 18S (95% CI) | n mice (n exp) |
| 0 (naïve) | 12.98 (12.58–13.37) | 7 (3) | |
| 7 | 1 | 2.56 (1.98–3.14)* | 7 (3) |
| 2 | 10.97 (8.62–13.31) | 5 (2) | |
| 3 | 11.95 (11.08–12.81) | 8 (3) | |
| 0 (naïve) | 13.06 (11.58–14.54) | 6 (2) | |
| 21 | 1 | 2.60 (0.40–4.80)* | 8 (2) |
| 2 | 7.40 (6.37–8.43)* | 8 (2) | |
| 3 | 12.10 (11.40–12.80) | 8 (2) |
BALB/c mice were immunized one, two, or three times at 7- or 21-d intervals and then killed 44 h after the final sporozoite injection. Naïve mice were also killed as controls. Total liver RNA was purified and real-time RT-PCR was performed to measure mouse GAPDH mRNA and P. yoelii 18S rRNA. The parasite burden is shown as the ΔCT (CT Py 18S rRNA – CT mouse GAPDH mRNA), which is inversely proportional to the parasite load. CI, confidence interval.
*
P < 0.0001 (unpaired t test comparison with unimmunized).