Fig. 1.

CyPD interacts with OSCP and is displaced by Bz-423. (A) Extracts and immunoprecipitates of BHM with anti-OSCP or b or d subunit antibodies were immunoblotted as indicated. Lane 1, mitochondria; lane 2, immunoprecipitates with OSCP (Left), b (Center), and d (Right) antibodies; lanes 3, IgG antibody. (B) Total cell extracts (Left) and complex V immunoprecipitates (Right) of mitochondria from cells either untreated (lane 1) or treated with scrambled siRNA (lane 2) or OSCP siRNA (lane 3) probed for F₁ α,β-subunits, OSCP, and CyPD. (C) Heart mitochondria were treated with the indicated concentrations of Bz-423 (μM), immunoprecipitated with anticomplex V antibodies, and immunoblotted with antibodies against β-subunit or CyPD. Ratio between CyPD and β-band intensities is reported (n = 3 ± SE). *P ≤ 0.02; **P = 0.0015, Student t test.