Fig. 2.

The 11,12-EET increases endfoot Ca²⁺ oscillations via TRPV4 channels. (A) Time course of an 11,12-EET-stimulated Ca²⁺ oscillation in an astrocytic endfoot. (Scale bar, 10 µm.) The total duration of the oscillation was 9.3 s. (B, a and B, c) The 11,12-EET (1 µM) increased the amplitude of endfoot Ca²⁺ oscillations (P < 0.05; n = 4 slices from three animals), but did not significantly increase oscillation frequency (B, b). (C) Preincubation of brain slices with HC-067047 (1 µM) prevented the effect of 11,12-EET on endfoot Ca²⁺ oscillations (n = 5 slices from two animals). Values were compared by paired t test.