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. 2013 Feb 19;110(15):6205–6210. doi: 10.1073/pnas.1215543110

Fig. 1.

Fig. 1.

BIK1 interacts with the PEPR1 KD. (A) BIK1 interacts with PEPR1 KD in yeast. Serial dilution of yeast cells containing the indicated plasmids were spotted on the indicated medium for lacZ and His reporter assays (four independent experiments). PEPR1-tKD, a prey plasmid containing a truncated PEPR1 KD (amino acids 1025–1123); BIK1, a bait plasmid containing full-length BIK1; pGADT7, empty prey plasmid; pGBKT7, empty bait plasmid; SD/TL−, SD medium lacking tryptophan and leucine; SD/TLH−, SD medium lacking tryptophan, leucine, histidine, and adenine; 3-AT, 3-amino-1,2,4-triazole. The center panel contains X-gal in the medium. (B) BIK1 interacts with the intact PEPR1 KD (amino acids 827–1123) in GST pull-down assay (three independent experiments). Anti-His immunoblot (IB) shows amounts of PEPR1-KD-His bound by the indicated GST-tagged proteins. Coomassie Brilliant Blue (CBB) staining indicates amounts of GST-tagged proteins. (C) BIK1 interacts with full-length PEPR1 in Arabidopsis protoplasts. BIK1-HA was expressed in WT Arabidopsis protoplasts, along with PEPR1-FLAG or BAK1-FLAG, immunoprecipitated with anti-HA antibody (α-HA IP), and the bound protein was detected by immunoblot with the indicated antibodies (four independent experiments).