Fig. 4.
(a) Micrographs showing varying degrees of differentiation of the C2C12 myoblasts under control, hypoxia, low glucose, or ischemia conditions. Myotubes are evident in both the control and hypoxia groups by day 6. (b) Survival of myoblasts over time under control, hypoxia, or low glucose was greater than under ischemia conditions. (c) Lactate dehydrogenase (LDH) levels released into the media by cells under experimental conditions (normalized to day 0 levels). The LDH levels of cells under ischemia conditions followed closely with the peak in cell death. The LDH levels in hypoxia and control conditions rose only when the cultures were primarily composed of myotubes. (d) A count of fully differentiated myoblasts under control, hypoxia, starvation, or ischemia conditions over a period of 14 days. Myoblasts were evident only after differentiation under control or ischemia conditions.