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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Curr Eye Res. 2012 May 11;37(8):671–677. doi: 10.3109/02713683.2012.675613

FIGURE 1.

FIGURE 1

Whole-cell patch clamp recordings of Cl− current in isolated rabbit LG acinar cells. Cells were initially perfused with external solution with high [Cl−] = 154 mM (control, n = 5). Addition of a cAMP-increasing cocktail of 100 μM IBMX and 10 μM forskolin (IBMX+Forsk, n = 5) elicited a strong Cl− current, suggesting the elicited current was mediated by CFTR, which is a cAMP-activated ATP-gated anion channel. Replacement of the external solution with low [Cl−] = 14 mM shifted the reversal potential from 0 mV to 41.95 ± 3.37 mV (n = 4). Data are presented as mean ± standard error of the mean (SEM). Student’s t-tests and/or ANOVA were performed by comparing data elicited from agent and control, and significant differences (p < 0.05) were observed at each holding potential.