Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Bone Miner Res. 2013 May;28(5):1203–1213. doi: 10.1002/jbmr.1822

Figure 2. Evidence of a common bone marrow monocyte progenitor for osteoclast, macrophages and dendritic cells in vitro.

Figure 2

A) Single cell cloning of BM-PIV cultured with M-CSF for 14 days or with GM-CSF for 8 days and IL-4 for 4 additional days in 96 U-bottom well plates. Microphotographs represent cells derived from a single colony of macrophage-like cells (top) or dendritic-like cells (bottom). Bar graphs on right indicate colony formation efficiency of single cells (93.5 ± 1.92) cultured with M-CSF (top) and single cells (93.5 ± 1.92) cultured with GM-CSF and IL-4 (bottom). Plating 5 cells per well resulted in a 100% cloning efficiency and no statistically significant differences were found. Data represent the average ± SD of 4 independent experiments. B) Comparative phenotypic analysis of PIV with cells derived from cultures under macrophage inducing (M-CSF) and DC inducing (GM-CSF+IL-4) conditions. Macrophage-like cells expressed the phenotype CD11b+ CD11c− F4.80+ MHC II−, while DC-like cells expressed the phenotype CD11b+ CD11c+ F4.80− MHC II+.