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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Eur J Nucl Med Mol Imaging. 2013 Jan 24;40(5):759–767. doi: 10.1007/s00259-012-2334-2

Fig. 1.

Fig. 1

Purification and characterization of TRC105-Fab. a The elution profile of TRC105-Fab from a Sephadex G-75 column. Arrowhead indicates the single fraction used for further in vitro and in vivo studies. b SDS-PAGE confirmed the purity of TRC105-Fab. Lane 1: molecular weight markers; lane 2: TRC105; lane 3: TRC105-Fab after Sephadex G-75 column purification. c HPLC analysis of TRC105 and TRC105-Fab. d Mass spectrometry of TRC105-Fab (~47.5 kDa) and its parent antibody TRC105 (~148 kDa). Doubly charged ion is also observed for TRC105 in the mass spectrum (i.e., 73,990 Da).