Fig. 6.
Immunofluorescence staining of 4T1 tumor, liver, kidney, and muscle tissue sections. TRC105 and AlexaFluor488-labeled goat anti-human IgG were used for CD105 staining (green). Rat anti-mouse CD31 antibody and Cy3-labeled donkey anti-rat IgG were used for CD31 staining (red). AlexaFluor350-TRC105-Fab (blue) was used to evaluate its overlay with CD105/CD31 staining. Hematoxylin staining was conducted to reveal the location of cell nuclei. Magnification: 200 ×. Scale bar: 50 µm.