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. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: Eur J Cancer. 2013 Jan 10;49(7):1761–1770. doi: 10.1016/j.ejca.2012.12.014

Fig. 1 — Knockdown of Pdcd4 down-regulates epithelial proteins and up-regulates mesenchymal proteins. (A) Expression of epithelial proteins: α-catenin and γ-catenin, mesenchymal proteins: N-cadherin and fibronectin, and GAPDH were examined in control (GEO-shLacZ and HT29-shLacZ) and Pdcd4 knockdown (GEO-shPdcd4 and HT29-shPdcd4) cells using Western blotting analysis. (B–I) GEO-shLacZ and GEO-shPdcd4 cells were orthotopically injected into mice as described in Materials and Methods. After 8 weeks, the tumor nodules in the cecum were collected, fixed, and embedded. Paraffin sections from GEO-shLacZ (B, D, F, H) and GEO-shPdcd4 (C, E, G, I) derived tumors were stained using IHC with antibodies against N-cadherin (B, C), β-catenin (D, E), E-cadherin (F, G), and α-catenin (H, I). Scale bar: 50 μm.

Fig. 1 — Knockdown of Pdcd4 down-regulates epithelial proteins and up-regulates mesenchymal proteins. (A) Expression of epithelial proteins: α-catenin and γ-catenin, mesenchymal proteins: N-cadherin and fibronectin, and GAPDH were examined in control (GEO-shLacZ and HT29-shLacZ) and Pdcd4 knockdown (GEO-shPdcd4 and HT29-shPdcd4) cells using Western blotting analysis. (B–I) GEO-shLacZ and GEO-shPdcd4 cells were orthotopically injected into mice as described in Materials and Methods. After 8 weeks, the tumor nodules in the cecum were collected, fixed, and embedded. Paraffin sections from GEO-shLacZ (B, D, F, H) and GEO-shPdcd4 (C, E, G, I) derived tumors were stained using IHC with antibodies against N-cadherin (B, C), β-catenin (D, E), E-cadherin (F, G), and α-catenin (H, I). Scale bar: 50 μm.