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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 5;1829(5):436–442. doi: 10.1016/j.bbagrm.2012.12.009

Fig. 2. Analyzing the role of miRNAs in the HNF-4A 3’-UTR with luciferase reporters.

Fig. 2

A. HepG2 cells were transfected with pmirGLO-HNF-4A 3’UTR (pmirGLO-HNF-4) or luciferase reporter vector alone (pmirGLO). B. The reporter (pmirGLO-HNF4) was cotransfected into the cells with siDicer I, siControl (siCtr) or alone (−). C. Cotransfections were carried out with reporter (pmirGLO-HNF4) and various miRNAs mimics as indicated. At 48 h after transfection, the cells were lysed and analyzed for Firefly and Renilla luciferase activity using a dual reporter assay system. The data shown are the ratio of the Firefly luciferase activity to the Renilla luciferase activity, and represent the mean ± SD obtained from three individual wells. The experiments were performed in triplicate. **p<0.01 versus pmirGLO-HNF4 (A), and versus the cells transfected with pmirGLO-HNF4 alone (−) (B and C).