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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 5;1829(5):436–442. doi: 10.1016/j.bbagrm.2012.12.009

Fig. 4. The effect of miRNAs on HNF-4α expression.

Fig. 4

HepG2 cells, seeded into 6-well plates, were transfected with 100 pmol of miRIDIAN miRNA mimics, negative control miRNA mimic (miR-Ctr) or were untreated (−). At 48 h or 72 h after transfection, mRNA and nuclear extracts were prepared, the expression levels of HNF-4α mRNA (A) and protein (B) were determined by RT-qPCR and Western blot, respectively. The bar graph in panel B summarizes the densitometric analyses of the effects of miRNAs on HNF-4α protein expression normalized by β-actin levels. The experiments were performed in triplicate, and presented as mean ± SD. *p<0.05 and **p<0.01 versus untreated control (−) or miR-Ctr.