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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 5;1829(5):436–442. doi: 10.1016/j.bbagrm.2012.12.009

Fig. 5. Over-expression of miR-34a, miR-34c-5p and miR-449a reduces HNF-4α binding to its target genes, ApoB, TTR, and α1-AT.

Fig. 5

EMSAs were performed to detect the effect of miRNAs on HNF-4α DNA binding activity. HepG2 cells were transfected with miRNA mimics as indicated. Nuclear extracts were prepared. The 32P-labelled oligonucleotide probes based on the HNF-4α-specific binding site in the promoter of ApoB, TTR or α1-AT genes were used [11]. Representative EMSAs of HNF-4α binding are shown. Histograms (right-hand panels) show densitometric analyses of HNF-4α-DNA complexes. Values represent mean ± SD of three separate experiments. ** p<0.01 versus untreated control (−).