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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 5;1829(5):436–442. doi: 10.1016/j.bbagrm.2012.12.009

Fig. 6. The inhibitory effect of miR-34a, miR-34c-5p and miR-449a on HNF-4α binding activity in intact cells.

Fig. 6

HepG2 cells were transfected with miRNA mimics as indicated. The abilities of HNF-4α to bind to the HNF-4α-specific binding site in the promoter of ApoB, TTR or α1-AT gene were determined by ChIP assay with either antibody against HNF-4α or mouse IgG (IgG, negative control). Chromatin-immunoprecipitated DNA was analyzed by qPCR with primers and probes specific for the HNF-4α-binding sites in ApoB, TTR or α1-AT gene promoter [20]. The control samples were set at 1. The results are mean ± SD (n =3) assayed in triplicate by qPCR. * p<0.05 and ** p<0.01 indicate that the value is significantly different from control.