Fig. 7. The transactivation potential of a HNF-4α target gene, TTR, is inhibited by miR-34a, miR-34c-5p and miR-449a.
The miRNA mimics were cotransfected into HepG2 cells with a luciferase construct containing the promoter region of TTR spanning nucleotides (191 to +5) or empty pGL4.11[luc2P] vector (pGL4). The data shown are the relative luciferase activity, the ratio of the Firefly luciferase activity to Renilla luciferase activity, and represent the mean ± SD of three independent experiments. **p<0.01 indicates a significant difference compared to controls without mimic treatment (−).