Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cell Microbiol. 2013 Jan 9;15(6):961–976. doi: 10.1111/cmi.12089

Fig. 8. rNdk blocks ATP-mediated cellular ROS and decreases extracellular ATP release.

Fig. 8

Fig. 8

A. Primary GECs were pre-treated with 5 μg/ml rNdk and incubated with 3mM ATP for 30 min, 3 and 6 h. rFimA (5 μg/ml) protein from P. gingivalis (Yilmaz et al., 2002) was used as negative control (not shown). The cells were measured for global ROS production by DCF staining as described in experimental procedures. (*) denotes statistical significance (P = 0.01 t-test). Data are representative of at least two experiments in duplicate.

B. HIGK cells and pannexin-1 deficient HIGK cells (via RNAi) were infected with P. gingivalis or Δndk strain and studied for extracellular ATP release during 24 h period. Extracellular ATP levels were measured by a luciferase-based assay. Light emission was measured as relative light units (rlu) and absorbance from replicate samples of uninfected cell supernatants was subtracted from each sample. Data are representative of at least two experiments in duplicate.