Fig. 7.
Effects of the mode of administration in BiVax vaccines. a Mice (3 per group) were immunized though different routes and the presence of antigen-specific CD8 T cells in blood was analyzed by tetramer analyses 7 days after the prime and the boost. **Statistical significance (P < .01) between i.v. and s.c. induced responses was observed after the boost. ns = no statistical significant difference was observed between i.v. and i.m. after the boost. b Mice (3 per group) were pretreated i.v. with either PBS (control), Pam2-Trp1 or poly-IC, and 5 h later received i.v. BiVax (poly-IC and Pam2-Trp1) poly-IC or Pam2-Trp1 (as indicated for each group). CD8 T-cell responses were measured by tetramer analysis on days 7 (post-prime) and 19 (post-boost). c Mice were injected i.v. with PBS, E749–57, or poly-IC and 5 h later received a second i.v. injection with BiVax (E749–57 + poly-IC), poly-IC or the E749–57 (as indicated for each group) and were boosted the same way 16 days later. The percentage of tetramer positive CD8 T cells was determined in blood 6 days after prime and boost (n = 3 mice per group)