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B cell autoantibody secretion. Purified B cells were incubated with medium alone (“none”) or with medium containing LPS for 8-10 days prior to assay of supernatant by ELISA for anti-laminin IgMa. Shown is the mean ± SD for OD405 for binding of undiluted supernatant to laminin coated wells minus binding to wells coated with diluent only. Positive control anti-laminin IgG H50-9 value was 1.59 ± 0.13, and anti-laminin IgMa A10C supernatant was 1.62 ± 0.12.
