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Procedure for confirmation of T0 transgenic plants
(a) PCR analysis of genome DNA of putative transgenic soybean plants using bar gene primers. The length of PCR production was 459 bp. M: DL2000 marker DNA; Ctr−: non-transformed soybean; Ctr+: plasmid DNA; (b, c) Events of the PCR positive lines were randomly selected and tested using LibertyLink strips (b) or GUS staining (c). +: positive transgenic soybean plant; −: negative transgenic soybean plant
