Table 1.
Regeneration rate and transient GUS staining of explants infected by Agrobacterium
Genotype | Shoot regeneration |
GUS staining |
|||||||
n 1 | Regeneration rate* (%) | n 2 | Strong |
Weak |
None |
||||
n 3 | Ratio (%) | n 3 | Ratio (%) | n 3 | Ratio (%) | ||||
Heihe 06 | 539 | 13.4±3.4jk | 40 | 1 | 2.5 | 17 | 42.5 | 22 | 55.0 |
Heihe 19 | 528 | 13.6±1.9jk | 50 | 1 | 2.0 | 20 | 40.0 | 29 | 58.0 |
Heihe 25 | 613 | 23.8±4.3hi | 55 | 0 | 0.0 | 19 | 34.5 | 36 | 65.5 |
Heihe 38 | 608 | 27.3±2.1gh | 52 | 4 | 7.7 | 18 | 34.6 | 30 | 57.7 |
Heihe 43 | 553 | 25.7±3.8gh | 40 | 4 | 10.0 | 25 | 62.5 | 11 | 27.5 |
Heihe 48 | 452 | 39.8±6.5f | 59 | 0 | 0.0 | 24 | 40.7 | 35 | 59.3 |
Hefeng 25 | 289 | 21.1±7.1hi | 37 | 9 | 24.3 | 16 | 43.2 | 12 | 32.4 |
Hefeng 35 | 370 | 13.5±2.6jk | 40 | 11 | 27.5 | 13 | 32.5 | 16 | 40.0 |
Heinong 35 | 604 | 17.1±3.6ij | 40 | 6 | 15.0 | 17 | 42.5 | 17 | 42.5 |
Heinong 37 | 12 102 | 52.2±1.1de | 44 | 9 | 20.5 | 16 | 36.4 | 19 | 43.2 |
Heinong 44 | 884 | 31.8±7.2g | 40 | 4 | 10.0 | 15 | 37.5 | 21 | 52.5 |
Heinong 48 | 374 | 10.2±3.6k | 40 | 4 | 10.0 | 21 | 52.5 | 15 | 37.5 |
Heinong 51 | 557 | 8.4±3.2k | 40 | 7 | 17.5 | 20 | 50.0 | 13 | 32.5 |
Heinong 58 | 2 027 | 58.7±1.9d | 41 | 4 | 9.8 | 19 | 46.3 | 18 | 43.9 |
Ha 03-3 | 190 | 9.0±2.7k | 40 | 0 | 0.0 | 6 | 15.0 | 34 | 85.0 |
Zhonghuang 30 | 400 | 20.8±5.1hi | 39 | 0 | 0.0 | 22 | 56.4 | 17 | 43.6 |
Zhonghuang 39 | 340 | 24.1±4.4h | 40 | 4 | 10.0 | 15 | 37.5 | 21 | 52.5 |
Tianlong 1 | 437 | 80.7±4.3ab | 42 | 26 | 61.9 | 11 | 26.2 | 5 | 11.9 |
Yuechun 04-5 | 898 | 75.4±3.2b | 40 | 16 | 40.0 | 18 | 45.0 | 6 | 15.0 |
Yuechun 03-3 | 355 | 51.5±4.7e | 40 | 18 | 45.0 | 16 | 40.0 | 6 | 15.0 |
Jack | 337 | 82.8±3.3a | 41 | 24 | 58.5 | 15 | 36.6 | 2 | 4.9 |
Williams 82 | 356 | 67.7±4.7c | 41 | 23 | 56.1 | 18 | 43.9 | 0 | 0.0 |
| |||||||||
Average | 34.9 | 19.5 | 40.7 | 39.8 |
Regeneration rate was expressed as mean±standard deviation; means were compared using Fisher’s least significant difference test, and different small letters showed significant difference (P<0.05)
n 1: number of explants infected; n 2: total number of explants for GUS staining; n 3: number of explants