Fig. 1.

Effects of Bu/Cy treatment on histology of the major internal organs

(a, b) The lungs; (c, d) The spleen; (e, f) The kidney; (g, h) The heart; (i, j) The stomach; (k, l) The liver; (m, n) The pancreas; (q, r) The ovary on Day 30; (s, t) The ovary on Day 60. H&E stainings of samples from Bu/Cy-treated mice were shown in (b), (d), (f), (h), (j), (l), (n), (r), and (t), and those from age-matched untreated mice were in (a), (c), (e), (g), (i), (k), (m), (q), and (s). There were thinner uterine walls and vaginal walls in Bu/Cy-treated mice (o, right) compared with the age-matched controls (o, left). The ovary of Bu/Cy-treated mice (p, right) was smaller than that of the controls (p, left). There was a significant reduction of primordial and primary follicles in Bu/Cy-treated mice (r) relative to age-matched untreated controls (q); There was a marked loss of follicles and mild fibrosis in part of interstitial area (arrow) in Bu/Cy-treated mice (t) compared with the controls (s)