Fig. 2.

Effect of Bu/Cy treatment on different follicle types

On Day 30, there was a significant reduction (P<0.01) of primordial and primary follicles in Bu/Cy-treated mice (hatched bar) compared with age-matched untreated mice (black bar). On Day 60, there was a significant reduction (P<0.01) of primordial, primary, secondary, and antral follicles in Bu/Cy-treated mice (white bar) compared with the controls (black bar). Each bar represents the mean±standard error of the mean (SEM) (n=5). ^** P<0.01 compared with untreated female mice at the age of three months