Figure 3.

Effects of GW501516 on cell proliferation and expression of multiple cell cycle regulatory genes in HPASMCs. (a) HAPSMCs were pretreated with GW501516 (GW, 3 μM) for 6 h prior to 36 h of PDGF-BB treatment (PDGF, 10 ng/mL). DNA synthesis was measured by BrdU incorporation assay. (b) mRNA levels of multiple cell cycle regulatory genes were determined in HPASMCs. Experiments were performed in triplicate and repeated a minimum of three times. ^# P < 0.01, ^† P < 0.05 versus control; *P < 0.05  versus PDGF-treated cells.