Fig. 12.

SN50, a blocker for NF-κB, treatment revealed the involvement of NF-кB in repressing the Prdx6 in HT22 cells during hypoxic stress. A and B: HT22 cells were treated with or without SN50 for 2 h followed by 200 μM CoCl₂. After 12 h, cytosolic or nuclear extracts and total RNA were isolated and proceed for Western analysis (A) and real-time PCR analysis (B). C: HT22 cells were transfected with pCAT-Prdx6 promoter construct and treated with SN50 for 2h followed by 200 μM CoCl₂ exposure. After 48 h, total proteins were isolated and proceed for CAT ELISA. Experiments were done 3 times, and values are represented as means ± SD. **P < 0.001, statistically significant difference.