Fig. 6.

Effects of long-term hypoxia on VEGF receptor expression. In homogenized endothelium-denuded fetal arteries, abundances of VEGF receptor 1 (Flt-1) and VEGF receptor 2 (Flk-1) were significantly increased by chronic hypoxia. Multiple bands on Western blots indicate different glycosylation states of the receptors. Values represent total of all glycosylation states for Flk-1. Because of the absence of the 230-kDa form of Flt-1 in hypoxic arteries, abundances of the 250-kDa form are compared. Standards on Western blots were prepared from mixed samples of normoxic adult arteries. Values are means ± SE; n = 6 in all experimental groups. *P < 0.05 by ANOVA.