Figure 4.

Effect of natural compounds on the DNMT transcript levels, viz., DNMT1, DNMT3a, and DNMT3b in human breast cancer cell lines. MCF 7 and MDA MB 231 cells were treated with EGCG, genistein, withaferin A, curcumin, resveratrol, guggulsterone (conc. of each compound used equals their IC₅₀ value) and decitabine (at conc. of 10 µM for MDA MB 231 cells and 12 µM for MCF 7 cells) for 96 hours and then real time polymerase chain reaction analysis was performed for the transcript levels of DNMT1 (A), DNMT3a (B), and DNMT3b (C).