Fig. 3.

Neuronatin silencing potentiates adipogenesis in inguinal white adipocytes. Expression levels of adipogenic marker genes PPARγ (A) and aP2 (B) in control and NE/Rosi-treated inguinal white adipocytes with or without neuronatin knockdown, were measured. Expression levels are given relative to those of the BA cultures. Statistical analysis of the siRNA effects were performed and indicated as described in methods. Neuronatin (C) and aP2 (D) levels were measured in inguinal white adipocytes in the presence of individual siRNAs from the siRNA pool targeting neuronatin. Values represent means ± SE of qPCR triplicates from a single experiment normalized to 18S expression relative to levels in Lipofectamine-treated adipocyte cultures. E: representative phase-contrast pictures of cells with and without neuronatin knockdown and with and without Oil red O staining.