Fig. 6.

Effect of neuronatin silencing on glucose uptake and glycogen synthesis in primary adipocytes. A: glucose uptake in inguinal white primary adipocytes with or without insulin stimulation and with or without neuronatin siRNA. Values represent means ± SE of 5 independent experiments, each conducted in triplicate. B: effect of neuronatin silencing on glycogen synthesis in control and insulin-stimulated inguinal white adipocyte cultures. Values represent means ± SE of 4 independent experiments, each conducted in triplicate (pmoles of glucose incorporated in glycogen per mg of protein). C: Western blots shown for P-GS, GS, P-GSK3β, and GSK3β with and without insulin stimulation of iWA are representative of 4 independent experiments. D: Western blot analysis is shown for GLUT1 with and without insulin stimulation; graphs represent means ± SE of 4 independent experiments. A representative blot is shown above the graph. Statistical analysis was conducted as described in methods. E: representative Western blots (minimum 3 independent experiments) are shown for principal insulin signaling intermediates upon neuronatin knockdown with or without NE/Rosi stimulation.