Fig. 5.
Reversibility of BSO effect on copper uptake by BSO washout. A: time-dependent BSO effect. 64Cu uptake in cells incubated in medium containing 250 μM BSO for 24–72 h, as indicated below each column. B: copper uptake following BSO treatment with or without drug washout. Cells were incubated in medium without or with 500 mM BSO for 48 h (1st 2 columns). For washout samples, BSO-treated cells were transferred to normal media 24 or 48 h before assay (3rd and 4th columns). *P < 0.05, significantly different value vs. control by Student's t-test, unpaired.