Fig. 6.

Restoration of ⁶⁴Cu uptake copper uptake in BSO- inhibited cells with GSH supplementation. A: cells were incubated for 48 h in media without or with 500 μM BSO (1st 2 bars) followed by overnight treatment with 1.5 mM GSH diethyl ester, a membrane permeable form of GSH which is hydrolyzed to release GSH in the cytoplasm (3rd and 4th bars) before ⁶⁴Cu uptake assay. B: ⁶⁴Cu uptake copper uptake and cellular GSH levels in cells treated with BSO or BSO and 1.5 or 4.0 mM GSHEt₂ (GSH). Controls (100%) are cells with no treatments. C: BSO effect on copper uptake in other cell lines expressing only endogenous CTR1. Cells were incubated for 48 h in medium with or without 500 μM BSO or with BSO treatment and overnight incubation in 1.5 mM GSHEt₂. Because GSHEt₂ was toxic to rat smooth muscle cells, no results are shown for those cells. * P < 0.05, significantly different value vs. control by Student's t-test, unpaired.