Fig. 7.

Effects of BSO treatment on copper uptake cells in which ATOX1 and CCS levels were varied. In A and B, cells were incubated in medium with or without 500 μM BSO for 48 h before the assay. A: cells were transfected with siRNA (targeted or control) indicated below each pair of columns 72 h before uptake assays. B: cells were transfected with expression or empty vector control plasmids 48 h before uptake assays, as indicated below each pair of columns. Note: experiments with ATOX1 knockdown have higher copper uptake than do control and CCS knockdown experiments, due to a higher level of hCTR1 expressed in the plasma membrane after knockdown (see experimental procedures and Fig. 2). *P < 0.05, significantly different value vs. no BSO treatment, Student's t-test, unpaired. In A, CCS: P = 0.133. In B, vector: P = 0.057.