Fig. 9.

Effects of BSO treatment on copper uptake by HCH-AAA mutant. A: ⁶⁴Cu uptake in HEK293 cells overexpressing wild type hCTR1 or mutant hCTR1 in which the carboxyl-terminal HCH (putative) metal binding motif is substituted with alanine residues (HCH-AAA). Cells were treated in media with or without added BSO (500 μM) for 48 h. B: ⁶⁴Cu uptake in HEK293 cells overexpressing the HCH-AAA mutant cells treated with BSO or BSO and 1.5 or 4.0 mM GSH-diethyl ester (GSH). Control (100%) cells had no BSO or GSHEt₂ treatment. *P < 0.05, significantly different value vs. no BSO treatment by Student's t-test, unpaired.