Table 1.

Copy number of GSH, ATOX1, CCS, and hCTR1 in HEK293 cells

	Molecule
	GSH (n = 3)	ATOX1 (n = 6)	CCS (n = 5)	hCTR1 (n = 5)
Copy #/cell	5.5 ± 1.9 × 109	3.2 ± 0.9 × 105	1.5 ± 0.3 × 104	1.6 ± 0.5 × 104

Values are means ± SE. hCTR1, human copper transporter 1. For GSH, copy number was calculated from cellular GSH concentrations determined using a luminescence-based glutathione assay kit (see experimental procedures). Cellular concentration of GSH was calculated based on a cell volume of 2 pl (see experimental procedures and Ref. 25). Levels of ATOX1, CCS, and hCTR1 were quantified using Western blots as described in experimental procedures.