TNF-α treatment suppressed UCP1 mRNA induction in 10T1/2 adipocytes in a time- and dose-dependent manner. A and B: time-dependent (A) and dose-dependent (B) effects of the TNF-α treatment on UCP1 mRNA induction in 10T1/2 adipocytes. Values are means ± SE for 3–4 wells. **P < 0.01, compared with ISO-stimulated 10T1/2 adipocytes. C: concentration-response curve for the suppression of UCP1 mRNA induction in 10T1/2 white adipocytes after 20 h of exposure to 0.3, 1, 3, 10, 20, 30, and 50 ng/ml of TNF-α. Each data point represents the mean of data from 3 wells with error bars representing the SE.