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Netrin-1 overexpression suppresses IR-induced drop in vascular density. Vascular density was determined by immunohistochemical staining of the vasculature using antibody against CD31 antigen. A dense network of vessels was seen in both cortex and outer medulla in sham-operated animals, which was drastically reduced after IR (2 wk) in WT animals. Netrin-1 overexpression suppressed IR-induced reduction in vascular density. Magnification ×660.
