Fig. 6.

IR-induced IL-6 expression was suppressed by netrin-1 overexpression in tubular epithelial cells. A: immunohistochemical localization of IL-6 was performed as described in materials and methods. No staining was seen in sham-operated kidney. Intense immunostaining was seen in tubular epithelial cells after IR, which was suppressed in netrin-1 transgenic mouse kidney. Magnification ×660. B: Western blot analysis of IL-6 expression in kidney of WT and netrin-1 transgenic animals subjected to sham operation or IR. Densitometric quantification is shown at the bottom. IR-induced increase in IL-6 expression was significantly suppressed in netrin-1 transgenic animals. *P < 0.05 vs. sham. #P < 0.001 vs. IR WT; n = 3.