Fig. 13.
Influence of DIDS upon I-V relationships for rabbit NBCe1-A. Oocytes were injected with cRNA encoding rabbit NBCe1-A, and I-V relationships were obtained by voltage clamp while the cells were sequentially exposed to 1) our ND96 solution, 2) a 33-mM HCO3− solution, and finally 3) 33-mM HCO3− solution that contained 200 μM DIDS (A). Averaged slope conductances measured between −20 and +20 mV for 6 cells are shown in B in which the results of certain paired t-tests are shown as horizontal bars (*P < 0.05). The composition of solutions used in this protocol is shown in Table 2.