Fig. 2.
Influence of the EGFP-tag upon I-V relationships for human vs. rabbit NBCe1-A. Oocytes were injected with cRNA encoding either rabbit NBCe1-A (A) rabbit NBCe1-A-EGFP (B), or human NBCe1-A-EGFP (C). I-V relationships were obtained by voltage clamp while the cells were sequentially exposed to ND96 solution and to our 33-mM HCO3− solution. Averaged slope conductances measured between −20 and +20 mV for at least 6 cells are shown in D, in which the results of certain one-tailed paired t-tests are shown as horizontal bars (*P < 0.05). The composition of solutions used in this protocol is shown in Table 2.