Table 6.
Component | Ca2+-free ND96, mM | ND96/SO32−, mM | 33 mM HCO3− |
---|---|---|---|
Na+ | 96 | 96 | 96 |
K+ | 2 | 2 | 2 |
Mg2+ | 1 | 1 | 1 |
Ca2+ | 0 | 0 | 0 |
Total cations, meq | 100 | 100 | 100 |
Cl− | 97.5 | 38.1 | 64.5 |
HCO3− | 0 | 0 | 33 |
SO32− | 0 | 26.4 | 0 |
HSO3− | 0 | 6.6 | 0 |
HEPES− | 2.5 | 2.5 | 2.5 |
Total anions, meq | 100 | 100 | 100 |
Mannitol | 0 | 33 | 0 |
HEPES (neutral) | 2.5 | 2.5 | 2.5 |
pH | 7.5 | 7.5 | 7.5 |
The 33-mM HCO3− solutions were equilibrated with 5% CO2/95% O2. Osmolarity of all solutions was ∼200 mosM.