Fig. 1.

Expression of NADPH oxidase (Nox) isoforms and their regulatory subunits in kidney cortex of Nox2 knockout (KO) and wild-type (WT) mice. A: RNA expression of Nox subunits in kidney cortex of WT controls and Nox2 KO mice after 8 wk of diabetes, as assessed by quantitative RT-PCR Ct values for transcripts expressed as (34-Ct), using 34 as a theoretical “no-expression” value for quantitative RT-PCR. Values are means ± SE; n = 4 for each group. Open bars, WT mice; solid bars, Nox2 KO mice. B: immunofluorescence imaging with anti-Nox2 antibody was performed on frozen kidney sections from mice after 8 wk of diabetes. Nox2 (red); nuclei (blue). Original magnification, ×63. WTDM, WT diabetic mice; KODM, Nox2 KO diabetic mice.