Fig. 2.

Effects of Nox2 deletion on urinary albuminuria, creatinine clearance, oxidative stress, and glomerular and tubulointerstitial pathology. Urinary albumin-to-creatinine ratio (A) and urine H₂O₂ excretion (C) were measured in diabetic mice 8 wk after induction of diabetes. WT mice, n = 30; WTDM mice, n = 24; Nox2 KO mice, n = 24; KODM mice, n = 38. Glomerular surface area (D) and mesangial matrix area (E) were measured by morphometric analysis of glomeruli in kidneys after 8 wk of diabetes. Creatinine clearance by HPLC (B) and tubulointerstitial area (F) using a point-counting system were measured after 20 wk of diabetes. Values are group means ± SE; n = 5 for each group in B, D, E, and F. *P < 0.05 vs. control group.