Fig. 1. Myosin IIA and B differentially localized in migrating cells.

(A) MDCK cell expressing both myosin IIA-mCherry and myosin IIB-GFP migrating on the pillar array. (B) The overlay image of myosin IIA (magenta) and IIB (green) with arrows indicating the directions and magnitudes of traction force exerted by the cell. Scale bar: 10 µm. (C) Fluorescence intensity profile of myosin IIA (magenta) and IIB (green), and local traction force profile (bottom) along the migrating cell shown in A and B.