Fig. 2.

Level of expression of RF transporter hRFVT-1 and hRFVT-3 proteins in pre- and postconfluent Caco-2 cells. Total proteins (60 μg) isolated from pre- and postconfluent Caco-2 cells were separated on 4–12% minigels and electroblotted onto a polyvinylidene difluoride membrane and probed with specific anti-hRFVT-1 (A) and anti-hRFVT-3 (B) polyclonal antibodies. The LI-COR detection system was used to detect immunoreactive bands and determine specific band intensity. Values are means ± SE from ≥3 independent sample preparations from different batches of cells. *P < 0.01, **P < 0.05.