Fig. 4.

Level of expression of hRFVT-1 and hRFVT-3 heterogeneous nuclear RNA (hnRNA) in pre- and postconfluent Caco-2 cells. A and B: real-time PCR was performed using hRFVT-1 and hRFVT-3 hnRNA gene-specific primers (Table 1) and RNA from pre- and postconfluent Caco-2 cells. Values are means ± SE of ≥3 independent determinations from 3 separate samples. C: activity of full-length SLC52A3 (hRFVT-3) promoter in pGL3-basic vector was determined following transient transfection into Caco-2 cells. Luciferase activity was determined in pre- and postconfluent Caco-2 cells and normalized relative to activity of simultaneously expressed Renilla luciferase. Values are means ± SE from ≥3 independent sets of determinations. *P < 0.01, **P < 0.05.