Table 1.

Combination of primers used to amplify the respective genes by real-time PCR, semiquantitative PCR, and cloning of the promoters

	Primers (5′-3′)
Gene	Forward	Reverse
	PCR primers
hRFVT-1	AAAAGACCTTCCAGAGGGTTG	AGCACCTGTACCACCTGGAT
hRFVT-3	CCTTTCCGAAGTGCCCATC	AGAAGGTGGTGAGGTAGTAGG
Human β-actin	AGCCAGACCGTCTCCTTGTA	TAGAGAGGGCCCACCACAC
rRFVT-1	AGCTACCTGTAGTGGTGA	CTCAGCCCCTGAACCA
rRFVT-3	TAAGGAAGATCACGGGCACCTC	GTCATCCAACTGGCCAACACAG
18S	GGGAGGTAGTGACGAAAAATAACAAT	TTGCCCTCCAATGGATCCTC
	hnRNA primers
hRFVT-1	GACCTGCCTGTGAC	AACCCTCTGGAAGG
hRFVT-3	TGACATCGCACAGG	CCTGCTGTTGATCTG
Human β-actin	TTCCTGGGTGAGTGGAG	GGACTCCATGCCTGAGAG
rRFVT-1	CTTTAGGCCAGCTTCTTG	AAAATGCGAGTGGACAG
rRFVT-3	TGGTGTTCTGCCCTCAG	GGGCCTGTGTTTGTAGC
Rat β-actin	CTGCTCTTTCCCAGATGAG	CTCATAGATGGGCACAGTG
	Primers used for cloning of hRFVT-3 promoter
hRFVT-3	CGACGCGTTTGATCAAATCCTGGTTG	CCGCTCGAGCTTCTTCCTTCTAGTACAAAGC

hRFVT and rRFVT, human and rat riboflavin (RF) transporters, respectively; hnRNA, heterogeneous nuclear RNA.