Fig. 8.

Viability of cultures treated with pH 4 acidified media assessed by Trypan blue exclusion. Cells were assessed for cell death by Trypan blue exclusion after 3, 6, and 24 h in culture with and without treatment with acidified media. The average percentage of dead cells observed in untreated cells after 3 h in culture was 2.1 vs. 5.0% after treatment with acidified media for 30 and 60 min (P < 0.05). The percentage of observed dead cells was not significantly different in untreated and treated cells after 6 and 24 h in culture.