Figure 4. A dividing fibroblast at a 2D-3D matrix interface captured in a DIC time-lapse series.
The 2D-adherent fibroblast as outlined with the white line at T0 retracted adhesions from the flat surface while rounding up for division. While the cell retracted completely from the flat surface it translocated perfectly centered over a NW island where it divided. A daughter cell, which was pushed out from the NW island (black arrowhead at 45 min) translocated to a free surface spot (white asterisk) where the mother cell previously had the smallest adhesive area (Suppl. Movie M4). Scale bar 5 μm.